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CTC

Detection of Circulating Tumor Cells
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Application

The assay is designed to robustly capture, enumerate, and obtain cytology data of circulating tumor cells (CTC) in the blood. It is applicable for assessing prognosis, treatment efficacy, and manage treatment decision for most cancer types including breast cancer, prostate cancer, and colorectal cancer.

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“Liquid Biopsy” of Circulating Tumor Cells

Circulating tumor cells (CTC) are the cells shed from a primary tumor into the blood stream and subsequently metastasize to various locations in the body. Microfilter device has been developed providing a non-invasive mean to capture CTC in the blood stream, which is promising to become a surrogate of the traditional tissue biopsy. CTC counts has been shown a significant independent predictor of progression-free survival (PFS) and overall survival (OS) in patients with metastatic breast, colorectal, or prostate cancer. Further characterization of CTC based on its cytology and molecular approach would gain insight of tumor biology which would pave the way for optimized treatment for each patient.

Test Description

Blood sample is filtered through a polymer microfilter with uniformed pore size and high porosity (Figure below) using an automatic pump to capture circulating tumor cells. CTC are generally bigger and more rigid than the blood cells. Therefore, the process can effectively enrich the CTC rapidly and effectively. Furthermore, the enrichment does not depend on antibody capture and works with both viable and fixed cells.

Specimen Requirement

Patient Preparation - If the patient is undergoing doxorubicin therapy, allow at least 7 days after a dose of doxorubicin before blood draw.

Specimen Preparation – Collect 10 mL blood in CellSave preservative tube or EDTA tube.

Storage/Transport Temperature - Room temperature. Do not refrigerate or freeze.

Stability - Ambient: 4 days